Device

Part:BBa_K2074005:Design

Designed by: Tong Tong   Group: iGEM16_FAFU-CHINA   (2016-10-17)


Cry10Aa+2A+Cyt1 expressed in C. reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 251
    Illegal NotI site found at 1023
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 687
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 836
    Illegal NgoMIV site found at 878
    Illegal NgoMIV site found at 935
    Illegal NgoMIV site found at 1439
    Illegal NgoMIV site found at 2115
    Illegal NgoMIV site found at 2771
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 857
    Illegal BsaI.rc site found at 3290
    Illegal SapI.rc site found at 1579


Design Notes

none


Source

HSP70A-RBCS2 promoter cloned from Chlamydomonas reinhardtii. Cry10Aa and Cyt1 cloned from Bacillus thuringiensis serovar israelensis strain BRC-LLP29, codon optimal for Chlamydomonas reinhardtii. Extended FMDV 2A peptides cloned from FMDV, codon optimal for Chlamydomonas reinhardtii.

References